1Genetic Epidemiology Research Institute, University of California, Irvine, CA, USA.
2Department of Life Science and Technology, Pai Chai University, Daejeon, Korea.
3Division of Human Genetics, Department of Pediatrics, University of California, Irvine, CA, USA.
4Department of Occupational and Environmental Medicine, Eulji University Hospital, Daejeon, Korea.
Copyright © 2012 The Korean Society for Preventive Medicine
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mt-Co 1 100 | mt-Co 1 200 | mt-Co 1 400 | b-actin 100 | b-actin 200 | b-actin 400 | ||
---|---|---|---|---|---|---|---|
Total | n1 | 66 | 67 | 64 | 45 | 16 | 12 |
Mean | 19.76 | 20.41 | 23.93 | 27.87 | 28.92 | 28.86 | |
SD | 1.53 | 2.01 | 2.46 | 1.03 | 0.95 | 1.13 | |
Source2 (p-value) | 0.23 | 0.64 | 0.18 | 0.66 | 0.07 | 0.07 | |
Fingernail | n | 35 | 32 | 31 | 18 | 6 | 6 |
Mean | 19.94 | 20.28 | 24.36 | 27.94 | 29.47 | 29.50 | |
SD | 1.76 | 1.62 | 2.43 | 0.71 | 0.47 | 0.09 | |
Toenail | n | 31 | 35 | 33 | 27 | 10 | 6 |
Mean | 19.54 | 20.51 | 23.52 | 27.81 | 28.58 | 28.22 | |
SD | 1.22 | 2.32 | 2.45 | 1.21 | 1.0 | 1.35 | |
Collections3 (p-value) | 0.56 | 0.89 | 0.75 | 0.40 | 0.90 | 0.84 | |
1st collection | n | 23 | 22 | 22 | 12 | 6 | 4 |
Mean | 20.03 | 20.48 | 24.21 | 27.62 | 29.05 | 29.09 | |
SD | 1.59 | 1.67 | 2.66 | 1.05 | 1.09 | 1.24 | |
2nd collection | n | 22 | 22 | 21 | 16 | 7 | 5 |
Mean | 19.67 | 20.50 | 23.63 | 27.78 | 28.79 | 28.88 | |
SD | 1.70 | 2.40 | 2.30 | 1.00 | 0.63 | 0.93 | |
3rd collection | n | 21 | 23 | 21 | 17 | 3 | 3 |
Mean | 19.54 | 20.24 | 23.94 | 28.13 | 28.97 | 28.53 | |
SD | 1.30 | 1.97 | 2.50 | 1.06 | 1.56 | 1.64 | |
Age group3 (p-value) | <0.001 | 0.01 | 0.009 | <0.001 | 0.12 | 0.28 | |
Teens | n | 22 | 22 | 21 | 12 | 1 | 1 |
Mean | 20.58 | 21.17 | 24.90 | 28.39 | 29.76 | 29.46 | |
SD | 1.42 | 1.43 | 2.15 | 0.65 | . | . | |
Fourties | n | 24 | 23 | 23 | 20 | 7 | 4 |
Mean | 18.85 | 19.49 | 22.74 | 28.19 | 29.35 | 29.51 | |
SD | 1.02 | 1.31 | 1.79 | 0.76 | 0.41 | 0.41 | |
Seventies | n | 20 | 22 | 20 | 13 | 8 | 7 |
Mean | 19.94 | 20.60 | 24.28 | 26.89 | 28.44 | 28.41 | |
SD | 1.65 | 2.69 | 2.94 | 1.06 | 1.11 | 1.3 | |
Family2 (p-value) | 0.03 | <0.001 | <0.001 | 0.20 | 0.79 | 0.38 | |
Faimily 1 | n | 35 | 34 | 32 | 30 | 12 | 9 |
Mean | 19.36 | 19.58 | 22.46 | 27.71 | 28.88 | 28.69 | |
SD | 1.55 | 1.18 | 1.77 | 0.92 | 1.09 | 1.27 | |
Family 2 | n | 31 | 33 | 32 | 15 | 4 | 3 |
Mean | 20.20 | 21.26 | 25.41 | 28.18 | 29.04 | 29.39 | |
SD | 1.42 | 2.32 | 2.18 | 1.20 | 0.33 | 0.17 |
Blank control | Blood (+) control | Nail 1 | Nail 2 | Nail 3 | |
---|---|---|---|---|---|
Location 1 | >30 | 9.07 | 13.78 | 13.23 | 10.69 |
Location 2 | Not detected | 9.87 | 14.58 | 14.23 | 11.52 |
RCN (%)1 of location 1 | 100 | 3.82 | 5.59 | 32.53 | |
RCN (%) of location 2 | 100 | 3.82 | 4.86 | 31.86 | |
Mean of RCN (%) of two loci | 100 | 3.82 | 5.22 | 32.19 |
Method of quantification | Mean | SD | |
---|---|---|---|
Total | OD reading | 9.82 | 17.58 |
Picogreen reading | 0.42 | 0.31 | |
Toenail | OD reading | 6.77 | 2.77 |
Picogreen reading | 0.48 | 0.39 | |
Fingernail | OD reading | 12.94 | 24.67 |
Picogreen reading | 0.35 | 0.19 |
mt-Co 1 100 | mt-Co 1 200 | mt-Co 1 400 | b-actin 100 | b-actin 200 | b-actin 400 | ||
---|---|---|---|---|---|---|---|
Total | n |
66 | 67 | 64 | 45 | 16 | 12 |
Mean | 19.76 | 20.41 | 23.93 | 27.87 | 28.92 | 28.86 | |
SD | 1.53 | 2.01 | 2.46 | 1.03 | 0.95 | 1.13 | |
Source |
0.23 | 0.64 | 0.18 | 0.66 | 0.07 | 0.07 | |
Fingernail | n | 35 | 32 | 31 | 18 | 6 | 6 |
Mean | 19.94 | 20.28 | 24.36 | 27.94 | 29.47 | 29.50 | |
SD | 1.76 | 1.62 | 2.43 | 0.71 | 0.47 | 0.09 | |
Toenail | n | 31 | 35 | 33 | 27 | 10 | 6 |
Mean | 19.54 | 20.51 | 23.52 | 27.81 | 28.58 | 28.22 | |
SD | 1.22 | 2.32 | 2.45 | 1.21 | 1.0 | 1.35 | |
Collections |
0.56 | 0.89 | 0.75 | 0.40 | 0.90 | 0.84 | |
1st collection | n | 23 | 22 | 22 | 12 | 6 | 4 |
Mean | 20.03 | 20.48 | 24.21 | 27.62 | 29.05 | 29.09 | |
SD | 1.59 | 1.67 | 2.66 | 1.05 | 1.09 | 1.24 | |
2nd collection | n | 22 | 22 | 21 | 16 | 7 | 5 |
Mean | 19.67 | 20.50 | 23.63 | 27.78 | 28.79 | 28.88 | |
SD | 1.70 | 2.40 | 2.30 | 1.00 | 0.63 | 0.93 | |
3rd collection | n | 21 | 23 | 21 | 17 | 3 | 3 |
Mean | 19.54 | 20.24 | 23.94 | 28.13 | 28.97 | 28.53 | |
SD | 1.30 | 1.97 | 2.50 | 1.06 | 1.56 | 1.64 | |
Age group |
<0.001 | 0.01 | 0.009 | <0.001 | 0.12 | 0.28 | |
Teens | n | 22 | 22 | 21 | 12 | 1 | 1 |
Mean | 20.58 | 21.17 | 24.90 | 28.39 | 29.76 | 29.46 | |
SD | 1.42 | 1.43 | 2.15 | 0.65 | . | . | |
Fourties | n | 24 | 23 | 23 | 20 | 7 | 4 |
Mean | 18.85 | 19.49 | 22.74 | 28.19 | 29.35 | 29.51 | |
SD | 1.02 | 1.31 | 1.79 | 0.76 | 0.41 | 0.41 | |
Seventies | n | 20 | 22 | 20 | 13 | 8 | 7 |
Mean | 19.94 | 20.60 | 24.28 | 26.89 | 28.44 | 28.41 | |
SD | 1.65 | 2.69 | 2.94 | 1.06 | 1.11 | 1.3 | |
Family |
0.03 | <0.001 | <0.001 | 0.20 | 0.79 | 0.38 | |
Faimily 1 | n | 35 | 34 | 32 | 30 | 12 | 9 |
Mean | 19.36 | 19.58 | 22.46 | 27.71 | 28.88 | 28.69 | |
SD | 1.55 | 1.18 | 1.77 | 0.92 | 1.09 | 1.27 | |
Family 2 | n | 31 | 33 | 32 | 15 | 4 | 3 |
Mean | 20.20 | 21.26 | 25.41 | 28.18 | 29.04 | 29.39 | |
SD | 1.42 | 2.32 | 2.18 | 1.20 | 0.33 | 0.17 |
Blank control | Blood (+) control | Nail 1 | Nail 2 | Nail 3 | |
---|---|---|---|---|---|
Location 1 | >30 | 9.07 | 13.78 | 13.23 | 10.69 |
Location 2 | Not detected | 9.87 | 14.58 | 14.23 | 11.52 |
RCN (%) |
100 | 3.82 | 5.59 | 32.53 | |
RCN (%) of location 2 | 100 | 3.82 | 4.86 | 31.86 | |
Mean of RCN (%) of two loci | 100 | 3.82 | 5.22 | 32.19 |
OD, optical density.
mt-Co 1, mitochondrially encoded cytochrome C oxidase 1. Number are variable, since undetermined (>30) threshold cycles were excluded. Independent test between the two subgroups. ANOVA/Duncan, Scheffe for post-hoc comparison among the three subgroups.
RCN, relative copy number. Was calculated from the difference in the threshold cycles between each nail samples and the blood(+) control. The copy number of the blood control was considered to be 100%.